DNA

Part:BBa_K4051020

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-08)


PCRABCD

Entire perchlorate reductase island attached with stop codon and BioBrick in between.

Sequences BBa_K40517 to BBa_K4051020 stitched together with added stop codons and biobricks in between

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1318
    Illegal BamHI site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2816
    Illegal NgoMIV site found at 3277
    Illegal NgoMIV site found at 4777
    Illegal AgeI site found at 1127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4767
    Illegal SapI.rc site found at 4294


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Categories
Parameters
None